A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains
نویسندگان
چکیده
منابع مشابه
A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains.
INTRODUCTION: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. METHODS: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. RESULTS: The assay showed 100% specificity against 17 Leptospira strains with a limit ...
متن کاملDevelopment of Duplex PCR Assay for Detection and Differentiation of Typical and Atypical Melissococcus plutonius strains
Melissococcus plutonius is the causative agent of an important honeybee disease, European foulbrood (EFB). In addition to M. plutonius strains with typical characteristics (typical M. plutonius), we recently reported the presence of atypical M. plutonius, which are phenotypically and genetically distinguished from typical M. plutonius. Because typical and atypical M. plutonius may have differen...
متن کاملA Duplex PCR Assay for the Detection of Ralstonia solanacearum Phylotype II Strains in Musa spp.
Banana wilt outbreaks that are attributable to Moko disease-causing strains of the pathogen Ralstonia solanacearum (Rs) remain a social and economic burden for both multinational corporations and subsistence farmers. All known Moko strains belong to the phylotype II lineage, which has been previously recognized for its broad genetic basis. Moko strains are paraphyletic and are distributed among...
متن کاملA Reverse transcription-PCR assay for detection of type A influenza virus and differentiation of avian H7 subtype
Abstract : Avian influenza virus (AIV) infection is a major cause of influenza mortality in birds and can cause human mortality and morbidity. Although the risk of infection with avian influenza virus (AIV) is generally low for most people, the pathogenic virus can cross the species barrier and acquires the ability to infect and be transmitted among the human population; therefore the ra...
متن کاملRapid differentiation of cryptic species within Cryptococcus gattii by a duplex PCR assay.
Cryptococcus gattii consists of four cryptic species, VGI, VGII, VGIII, and VGIV. Herein, a duplex PCR assay using two primer pairs targeting the vacuolar membrane gene and the intergenic spacer region was developed. It successfully distinguished the cryptic species according to the distinct size of the amplicons.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Revista da Sociedade Brasileira de Medicina Tropical
سال: 2017
ISSN: 0037-8682
DOI: 10.1590/0037-8682-0364-2016